Coding

Part:BBa_K1779204:Design

Designed by: Xingyang Qiu   Group: iGEM15_CHINA_CD_UESTC   (2015-09-07)


Integration of twogenes, including RFP and laccase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 555
    Illegal AgeI site found at 667
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We designed primers with linker will RFP and laccase three genes linked by PCR of fusion


Source

RFP gene is obtained from the iGEM official bio bricks, which numbered BBa_E1010.

Laccase gene is obtained from the iGEM official bio bricks, which numbered BBa_K863005.

References